Biotech Studies

Isolation, identification and characterization of three new strains of Bacillus sphaericus as mosquito pathogen

Münevver Müge Çağal 1 ,Ali Umman Doğan 2 ,Fatih Çakar 3 ,Fikrettin Şahin 2

1 Bursa Technical University, Faculty of Engineering and Natural Sciences, Department of Bioengineering, 16310 Yıldırım, Bursa, Turkey
2 Yeditepe University, Faculty of Engineering and Architecture, Department of Genetics and Bioengineering, 34755 Kayışdağı, Istanbul, Turkey
3 University of Illinois, Department of Microbiology, Urbana Champaign, 61801 Urbana, Illinois USA
DOI : 10.38042/biost.2020.29.02.01 Viewed : 25 - Downloaded : 41 In the present study, three entomopathogenic strains of Bacillus sphaericus (MBI5, MBI6 and MBI7) were isolated from greenhouse pest and larval habitat of Istanbul, Turkey. All of the MBI groups tested in laboratory bioassays were able to kill larvae, but not pupae and adults of Culex spp. in water. The most efficient larvicidal activity of the strains was found in the presence of two toxin genes, binA and binB for MBI5, MBI6 and MBI7 strains. Each B. sphaericus strains identified in this study were characterized as unique and novel in terms of FAME profiles and 16S rRNA sequencing data. The results of this study suggest that the three strains of B. sphaericus may be new sources of potential biocontrol agent of mosquito. Keywords : Bacillus sphaericus Mosquito Biocontrol