Biotech Studies 2000, Vol 9, Num, 1     (Pages: 128-132)

EFFECT OF TEMPERATURE ON Agrobacterium tumefaciens MEDIATED TRANSFORMATION OF TOBACCO

Turgay ÜNVER 1 ,Khalid M. KHAWAR 2 ,İskender PARMAKSIZ 2 ,Leyla AÇIK 1

1 Gazi Üniv. Fen Edebiyat Fak. Biyoloji Böl 06500, Beşevler. Ankara
2 Ankara Üniv. Ziraat Fak. Tarla Bitkileri Böl. 06110. Dışkapı. Ankara
- Various abiotic factors play important role in plant development and production. Enviromental stress comes from biologic and abiotic factors. Temperature is an important factor than effects gene transfer process by Agrobacterium tumefaciens. Tobacco is generally used as a model plant in gene transfer studies. Tobacco variety Samsun was used in the study to find the effect of temperature and was found that 22 °C was the most effective temperature for the gene transfer studies. Keywords : Tobacco, Agrobacterium tumefaciens, temperature