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¹ Trakya Tarımsal Araştırma Enstitüsü, Edirne DOI : 10.21566/tarbitderg.280269 - Commercial hybrid rice has been utilized effectively around the world since 1975 but it is a new concept for Turkey. First step is to provide the male sterile lines to hybrid breeding system. Genetic and non-genetic mechanisms are available to exploit for male sterility. The aim of this study is identification of utilization of male sterility systems which Chemical Male Sterility (CHA), Thermogenic Genetic Male Sterility (TGMS) and Cytoplasmic Male Sterility for Turkey Ecosystem. This experiment were conducted two years in Edirne ecological conditions in 2013-2014. Chemical Hybridisation Agents (CHA) which salicylic acid, gibberellic acid, Ethephon were sprayed as a gametocide doses 1000 ppm at the booting stage on rice varieties which Paşalı, Halilbey, Osmancık-97. Male sterility was identified 22-49% after applied gametocide in cultivars. TGMS1 and TGMS2 cultivars have 89-96% male sterility in the uncontrolled field situation. CMS lines which V20A, 15A, 31A have been determined 100% male sterility. As a result, TGMS lines and applied doses gametocides are not efficient for male sterility but CMS system is efficient 100% in Trakya region. Japonica type commercial hybrid rice breeding appear possible in Turkey ecological condition using A, B and Restorer Lines. Keywords : CMS, male sterility, gametocide, hybrid rice, TGMS