CiteScore: 1.8
SCOPUS 2023
UN SDG
EVENTS
Biotech Studies 2017, Vol 26, Num, 1     (Pages: 111-126)

New Generation Genome Editing Techniques: ZFNs, TALENs, CRISPRs and Their Use in Plant Research

M. Aydın AKBUDAK 1 ,Kübra KONTBAY 1

1 Akdeniz Üniversitesi, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bolümü, An DOI : 10.21566/tarbitderg.323614 - ZFNs, TALENs and CRISPRs are the new generation genome editing tools functioning by binding a specified target region on DNA. Using one of these techniques, target genes could be edited by insertion/ deletions. It is also possible to replace nucleotides in target genes via homologous recombination. Compared to the current methods, new generation genome editing is faster, easier, more efficient and less expensive. This review covers how and where new generation genome editing techniques can be used, comparison one to another, and highlights applications in plant genetic engineering and plant breeding. Furthermore, the release of genome edited plants to the environment and current regulations in the world are discussed. It is predicted that improvements in binding and excising efficacy of new generation genome editing techniques would take the current research one step further, and would be a major aid to plant breeding that would lead to generating crop varieties with higher yield, quality and tolerance to biotic and abiotic stresses. Keywords : ZFN, TALEN, CRISPR, gene silencing, genome modification