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¹ Necmettin Erbakan University, Meram Faculty of Medicine, Depertman of Medical Biology, 42090, Konya, Türkiye DOI : 10.38042/biotechstudies.1273778 - Chrysin, a natural flavonoid, has a strong neuroprotective effect in many neurodegenerative diseases. Therefore, we aimed to investigate the neuroprotective effect of chrysin against bupivacaine-induced neurotoxicity in SH-SY5Y cells. According to the results of XTT analysis, the non-toxic concentration of chrysin was determined and the cells were treated with bupivacaine alone and together with this determined chrysin dose. According to the results of RT-qPCR analysis, the level of caspases increased in the group treated with only bupivacaine compared to the control group, while the expression of antioxidant enzymes decreased. When compared with the group treated with bupivacaine alone, it was determined that while the expression of caspases decreased in the group in which bupivacaine and chrysin were treated together, the expression of antioxidant enzymes increased. According to the ELISA results, SOD and CAT activities were decreased in the group treated with bupivacaine alone compared to the control group. SOD and CAT activities increased in the presence of chrysin treated with bupivacaine compared to the group treated with bupivacaine alone. The obtained data showed that chrysin may play a neuroprotective role by inducing the expression of antioxidant enzymes while inhibiting apoptosis against bupivacaine-induced neurotoxicity in SH-SY5Y cells. Keywords : Antioxidant Apoptosis Bupivacaine Chrysin Neurotoxicity