Abstract Full Text:PDF Recommended articles Mail to Author

¹ Department of Biotechnology, Multi Faculty Building (Phase-I), Gyan Path, HP University Campus, Summer Hill, Shimla 171 005 (HP), India
² Department of Biotechnology, Chandigarh College of Technology, Chandigarh Group of Colleges, Landran, Mohali 140307 (Punjab), India DOI : 10.38042/xx.xx - Butyrohydroxamic acid is a hydroxamic acid that has various biological and pharmacological applications. This study reports the bioconversion of butyramide and hydroxylamine to butyrohydroxamic acid with the help of amidase of Bacillus sp. APB- 6, which has amidotransferase activity. Optimal conditions for the reaction were determined as 100/1200 mM butyramide/hydroxylamine ratio, incubation time 5 hr, pH 9.5, temperature 55°C, and resting cell concentration of 1.578 mg dcw ml-1. Under these conditions, the complete conversion of butyramide to butyrohydroxamic acid was attained in a 50 ml flask scale. The batch reaction was preferred over fed-batch reaction for scaling up the process to a 1 L scale, and the reaction time was reduced by 30 minutes. The final product yield was 10.23 g butyrohydroxamic acid with 95% purity, volumetric productivity of 2.273 g/L/h and 1.44 g/g/h catalytic productivity. The amidase used in this study showed high amidotransferase activity along with the industrially relevant process for the production of butyrohydroxamic acid. The NMR spectrum of the recovered product confirmed its identity as butyrohydroxamic acid. Keywords : Butyrohydroxamic acid Amidotransferase activity Fed-batch Whole resting cell NMR