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¹ Tarla Bitkileri Merkez Araştırma Enstitüsü Müdürlüğü-Ankara
² Gaziosmanpaşa Üniversitesi, Ziraat Fakültesi Tarla Bitkileri Bölümü-Tokat - In this study, twenty-five lines selected from Tokak (PI 470281) barley landrace using molecular markers were investigated in field studies under Tokat Kazova conditions in Turkey. Harrington, a malting barley variety of Canada, and Tokak 157/37, a commonly grown malting barley variety in Turkey, were used as reference. PI 470281 lines were investigated in terms of food and feed quality properties such as crude ash, crude protein, ADF, NDF contents, various mineral contents such as P, K, Ca, Mg, Fe, Cu, Mn, Zn, Cr and some agronomic traits such as days to flowering and maturity and plant height, number of seeds per head, spike length, 1000 seed weight, test weight, grain yield, biological yield and harvest index. PI 470281 lines investigated showed significant variations in terms of their agronomic, feed and food quality properties. Most of the lines had higher grain yields than Tokak 157/37 and lower than Harrington. 1000-seed weights and test weights for the lines investigated varied from 40 to 51g and 64 to 69 kg, respectively. Their ADF and NDF contents were higher than or similar to those of the reference cultivars. The crude protein contents varied from 12% to 14%and were significantly different among purelines. Significant variations were found among PI 470281 lines in terms of mineral contents. Fe, Cu, and Ca concentrations of pure lines were higher than or similar to those of the reference values. As a result, Tokak barley landrace has high genetic variations, and purelines developed from this variety has variations that could be used in breeding for agronomic, feed, and food quality properties. Keywords : Mineral element content, 1000-seed weight, acid detergent fiber, crude protein, crude ash