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¹ Kahramanmaraş Sütçü İmam Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bl., Kahramanmaraş - In this study, effects of different temperature values (5, 10, 15, 20 and 25 OC) on the germination of I. tinctoria and I. buschiana seeds, which have different vegetation periods, were investigated. Effects of vegetation periods on the ratio and components of seed oil were also analyzed by using GC-MS. While different vegetation periods were not effected the germination of I. tinctoria seeds, germination of I. buschiana seeds were differed according to vegetation periods. Late germination was observed in the seeds of both species at 5 OC, therefore germination ratio was found to be low in this temperature value. Effects of different concentrations of GA₃, Putresin and ALA on the germination ratio at 5 OC was investigated. Germination of I. buschiana and I. tinctoria seeds were increased by GA₃ with concentrations of 50 ppm and 75 ppm, respectively, at 5 OC. The ratio and components of seed oil were found to be similar in different vegetation periods. Keywords : Isatis, oil, germination, GA₃, ALA, Putrescine