Biotech Studies 2016, Vol 25, Num, 1     (Pages: 232-236)

Obtaining of Doubled Haploid Plants from Bread Wheat (Triticum aestivum L.) Hybrid Populations by Anther Culture Technique

İsmet BAŞER ,Kayıhan Z. KORKUT ,Oğuz BİLGİN ,Alpay BALKAN

1 Namık Kemal Üniversitesi Ziraat Fakültesi Tarla Bitkileri Bölümü, Tekirdağ DOI : 10.21566/tarbitderg.280493 Viewed : 935 - Downloaded : 864 The objectives of this study were to determine for response rate of anther culture response in wheat hybrid populations In addition to this, response of seventy bread hybrid wheat populations in their early uninucleate period to anter culture were determined by using W14F medium. In the study, the number of calluses which developed from anthers, the number of albino plantlets, the number of green plantlets which transferred to test tubes, the number of green plants which transferred to the soil and the number of spontaneous double haploid plant were determined. Based on the results of significance test, the differences between genotypes in the hybrid populations for all investigated characters were found statistically significant. Green plantlets number, callus number, green plantless number in tubes, plant number in soil and spontaneous double haploid plant rate were changed between 1.0 to 25.25 unit, 128.75-2.25 unit, 1.0-24.00 unit, 0.0-17.50 unit and 55.00-0.00 %, respectively. The maximum number of plantlets, which transferred to the greenhouse, obtained from Sagittario x Pehlivan and Sagittario x Luzanovka hybrids population 17.30 units and 9.50 units, respectively. Keywords : Anther, bread wheat population, spontaneous doubled haploid, albino, callus